Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats.
2019
Аутори:
Stanisavljević, SuzanaČepić, Aleksa
Bojić, Svetlana
Veljović, Katarina
Mihajlović, Sanja
Nikolovski, Neda
Jevtić, Bojan
Momčilović, Miljana
Lazarević, Milica
Mostarica Stojković, Marija
Miljković, Đorđe
Golić, Nataša
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with stronger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system.
Извор:
Scientific Reports, 2019, 9, 1, 918-Финансирање / пројекти:
- Изучавање гена и молекуларних механизама у основи пробиотичке активности бактерија млечне киселине изолованих са подручја западног Балкана (RS-173019)
- Ћелијски и молекулски механизми опоравка пацова од експерименталног аутоимунског енцефаломијелитиса (RS-173035)
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-173013)
DOI: 10.1038/s41598-018-37505-7
ISSN: 2045-2322
PubMed: 30696913
WoS: 000456956200008
Scopus: 2-s2.0-85060813602
URI
http://www.nature.com/articles/s41598-018-37505-7http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6351648
https://radar.ibiss.bg.ac.rs/handle/123456789/3264