Impact of the mesoporous silica SBA-15 functionalization on the mode of action of Ph3Sn(CH2)6OH
2019
Authors:
Edeler, DavidDrača, Dijana
Petković, Vladana
Natalio, Filipe
Maksimović-Ivanić, Danijela
Mijatović, Sanja
Schmidt, Harry
Kaluđerović, Goran N.
Document Type:
Article (Published version)
,
© 2019 Elsevier B.V.
Metadata
Show full item recordAbstract:
Herein appropriateness of nonfunctionalized mesoporous silica nanoparticles SBA-15 and functionalized with (3-chloropropyl)triethoxysilane (→ SBA-15~Cl) and (3-aminopropyl)triethoxysilane (→ SBA-15~NH2) on delivery of physically adsorbed Ph3Sn(CH2)6OH (Sn6) is evaluated. Fluorescent nanomaterial, bearing isatoic moiety, loaded with Sn6 (→ SBA-15~NF|Sn6) was used for cellular uptake study. The fluorescent nanomaterial is efficiently acquired and distributed into the cytoplasm of the cells even after 2 h of cultivation. According to the attained data, all SBA-15 materials loaded with Sn6 diminished cellular viability in dose dependent manner while carriers alone (SBA-15, SBA-15~Cl, SBA-15~NH2) did not show cytotoxicity against B16 cells. According to the MC50 values structural modification of SBA-15 did not improve the efficacy of tested drug. While progressive apoptosis was detected upon the treatment with SBA-15|Sn6, exposure of cells to SBA-15~NH2|Sn6 revealed extinguished apoptosis in time, accompanied with lower caspase activity. This effect is probably due to triggered autophagic process under the treatment with the SBA-15~NH2|Sn6, thus opposed to apoptosis. Presented results suggested that functionalization of SBA-15 was not beneficial for the efficacy of loaded drug, thus, all of them are almost equally efficient considering loaded Sn6 content. Importantly, functionalization of SBA-15 does have an influence on the mode of action and differentiation inducing properties.
Keywords:
SBA-15; Organotin(IV) compound; Apoptosis; Autophagy; Cell differentiationSource:
Materials Science and Engineering: C, 2019, 100, 315-322Funding / projects:
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
URI
https://www.sciencedirect.com/science/article/pii/S0928493118329175?via%3Dihubhttps://radar.ibiss.bg.ac.rs/handle/123456789/3302