Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala)
2019
Аутори:
Banjac, NevenaVinterhalter, Branka
Krstić Milošević, Dijana
Milojević, Jelena
Tubić, Ljiljana
Ghalawenji, Nabil
Zdravković-Korać, Snežana
Тип документа:
Чланак у часопису (Рецензирана верзија)
,
© 2019, Springer Nature B.V.
Метаподаци
Приказ свих података о документуАпстракт:
Brassica oleracea var. acephala is an important leafy vegetable that has been widely consumed as a high-nutrient, low-calorie food. Because of the plant’s biennial and self-incompatibility nature, biotechnological approaches are alternative way for propagation and breeding improvements. Since tissue culture studies have been focused in other B. oleracea representatives, the aim of the present study was to achieve effective regeneration protocol distinctive for collard greens, and evaluate the total phenolic content and antioxidant activity of regenerants. The effect of 3 cytokinins [thidiazuron (TDZ), 6-benzyladenine (BA) and 6-furfuryladenine (kinetin, KIN)] at increasing concentrations (0, 5, 7.5, 10, 20 or 30 µM) in combination with tenfold lower concentration of 1-naphtaleneacetic acid (NAA) (0, 0.5, 0.75, 1, 2 or 3 µM, respectively) on the regeneration from hypocotyl slices was studied. Histological analysis revealed the two regeneration pathways, somatic embryogenesis and shoot organogenesis, simultaneously occurred in the same explant, regardless of the cytokinin/NAA combinations used. The regeneration frequency of 95.9%, with 7.5 morphogenic structures regenerated per explant, and the healthy appearance of regenerated plants indicated the optimal combination 20 µM TDZ + 2 µM NAA. TDZ at 5 µM provided the high somatic embryo proliferation rate by generation of secondary embryos (7.79) along with the lowest rate of their abnormalities. Embryo-developed plants were successfully acclimatised (above 90%). The plants regenerated and proliferated on TDZ-containing media had higher total phenolic content that correlated with the highest free radical scavenging activity (IC50 = 19.09 µg ml− 1).
Кључне речи:
Adventitious shoots; Collard greens; Cytokinins; Regeneration; Somatic embryos; Somatic embryos proliferation; Total phenolics; Adventitious shoots; Collard greens; Cytokinins; Regeneration; Somatic embryos; Somatic embryos proliferation; Total phenolicsИзвор:
Plant Cell, Tissue and Organ Culture (PCTOC), 2019Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
URI
http://link.springer.com/10.1007/s11240-019-01595-6https://radar.ibiss.bg.ac.rs/handle/123456789/3310
Колекције
Институција/група
IBISSAPA:
Banjac, N., Vinterhalter, B., Krstić-Milošević, D., Milojević, J., Tubić, L., Ghalawenji, N., & Zdravković-Korać, S. (2019). Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). Plant Cell, Tissue and Organ Culture (PCTOC), DOI:10.1007/s11240-019-01595-6.Vancouver:
Banjac N, Vinterhalter B, Krstić-Milošević D, Milojević J, Tubić L, Ghalawenji N, Zdravković-Korać S. Somatic embryogenesis and shoot organogenesis from the hypocotyl slices and free radical scavenging activity of regenerants of collard greens (Brassica oleracea L. var. acephala). Plant Cell, Tissue Organ Cult. 2019;DOI:10.1007/s11240-019-01595-6.Related items
Showing items related by title, author, creator and subject.
-
Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact
Zdravković-Korać, Snežana; Ćalić-Dragosavac, Dušica D.; Uzelac, Branka; Janošević, Dušica A.; Budimir, Snežana; Vinterhalter, Branka; Vinterhalter, Dragan (Plant Cell Tissue and Organ Culture, 2008) -
Expression of the gene for ribosome-inactivating protein, SoRIP2, as a tool for the evaluation of somatic embryogenesis in spinach
Belić, Maja; Savić, Jelena; Tubić, Ljiljana; Devrnja, Nina; Ćalić, Dušica; Zdravković-Korać, Snežana; Milojević, Jelena (Plant Cell, Tissue and Organ Culture (PCTOC), 2017) -
Agrobacterium rhizogenes-mediated transformation of Gentiana utriculosa L. and xanthones decussatin-1-O-primeveroside and decussatin accumulation in hairy roots and somatic embryo-derived transgenic plants
Vinterhalter, Branka; Savić, Jelena; Zdravković-Korać, Snežana; Banjac, Nevena; Vinterhalter, Dragan; Krstić Milošević, Dijana (Industrial Crops and Products, 2018)