Приказ основних података о документу
Isolation and enrichment of mouse insulin-specific CD4+ T regulatory cells.
dc.creator | Nikolovski, Neda | |
dc.creator | Paunović, Verica | |
dc.creator | Stojanović, Ivana D. | |
dc.date.accessioned | 2019-05-16T12:09:22Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2019 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S002217591930047X?via%3Dihub | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/3347 | |
dc.description.abstract | Polyclonal T regulatory cells (Treg - CD4+CD25+CD127lowFoxp3+) are used in several protocols for the treatment of type 1 diabetes (T1D), multiple sclerosis and graft-versus host disease in clinical trials. However, general opinion is that autoantigen-specific Treg could be more efficient in autoimmunity suppression due to their direct effect on pathogenic autoantigen-specific effector T cells. This study describes isolation and expansion of insulin-specific Treg in vitro. Insulin-specific Treg are uniformly distributed in lymphoid tissues however their number is extremely low. To enrich the proportion of insulin-specific Treg, pure CD4+ cells were co-cultured with insulin B chain peptide-loaded dendritic cells, isolated from mice that develop T1D spontaneously - NOD mice. Insulin-specific CD4+ cell expansion peaked after 48 h of incubation and was in favour of Treg. These cells were then sorted using insulin peptide-loaded MHC class II tetramers and cultured in vitro for 48 h in the presence of TCR stimulators, TGF-β and IL-2. The proportion of gained insulin-specific cells with T regulatory phenotype (CD4+CD25highCD127lowGITR+FoxP3+) was in average between 93% and 97%. These cells have shown potent in vitro suppressive effect on T effector cells, produced IL-10 and TGF-β and expressed PD-1 and CD39. Further proliferation of these insulin-specific Treg required the presence of dendritic cells, anti-CD3 antibody and IL-2. This study provides new, reproducible experimental design for the enrichment and expansion of insulin-specific Treg that can be used for the cell-based therapy of autoimmunity. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173013/RS// | |
dc.relation | The Iacocca Family Foundation, Boston, MA, USA | |
dc.rights | restrictedAccess | |
dc.source | Journal of Immunological Methods | |
dc.subject | Expansion | |
dc.subject | Immunoregulation | |
dc.subject | Insulin-specific T regulatory cells (Treg) | |
dc.subject | Type 1 diabetes (T1D) | |
dc.subject | ex vivo protocol | |
dc.title | Isolation and enrichment of mouse insulin-specific CD4+ T regulatory cells. | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Пауновић, Верица; Стојановић, Ивана; Ђедовић, Неда; | |
dc.rights.holder | © 2019 Elsevier B.V. | |
dc.citation.volume | 470 | |
dc.identifier.doi | 10.1016/j.jim.2019.04.011 | |
dc.identifier.pmid | 31039339 | |
dc.identifier.scopus | 2-s2.0-85065012351 | |
dc.identifier.wos | 000483322000008 | |
dc.citation.apa | Đedović, N., Paunović, V., & Stojanović, I. (2019). Isolation and enrichment of mouse insulin-specific CD4+ T regulatory cells. Journal of Immunological Methods, 470, 46–54. | |
dc.citation.vancouver | Đedović N, Paunović V, Stojanović I. Isolation and enrichment of mouse insulin-specific CD4+ T regulatory cells. J Immunol Methods. 2019;470:46–54. | |
dc.citation.spage | 46 | |
dc.citation.epage | 54 | |
dc.type.version | publishedVersion | |
dc.citation.rank | M22 |