Ćelijska smrt u ozračenim ćelijama kancera prostate analizirana protočnom citometrijom

Abstract

Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.

Uprkos značajnom napretku u hemoterapiji kancera, radioterapija ostaje metod izbora u tretmanu metastaziranog kancera prostate. Ovaj rad predstavlja kvantitativnu analizu efekata 60Co gama zračenja na ćelijski rasti i ćelijsku smrt PC-3 ćelijske linije humanog kancera prostate, pri čemu je praćena vremenska (2-72h) i dozna zavisnost (2-20 Gy). Ozračene PC-3 ćelije su uglavnom umirale nekrozom u kasnijem vremenskom intervalu (72h), dok je apoptoza bila zanemarljiva. Vrednost EC50 odnosno 50% citotoksičnosti nije dostignuta primenjenim dozama, ali je ustanovljena značajna inhibicija ćelijskog rasta, sa vrednošću IC50 od 10.4 Gy. Zaključeno je da povećanje doze može imati značajan citostatički efekat ali da je za kompletno odstranjivanje metastaziranog kancera prostate neophodno uvođenje novih citotoksičnih agenasa ili radiosenzitera kao adjuvanata.