Antioxidative defense system in liver of adult and subadult Bufotes viridis frogs
2017
Аутори:
Nasia, MohammedGavrilović, Branka
Krizmanić, Imre
Gavrić, Jelena
Prokić, Marko
Borković Mitić, Slavica
Pavlović, Slađan
Despotović, Svetlana
Radovanović, Tijana
Saičić, Zorica
Тип документа:
Конференцијски прилог (Објављена верзија)
,
Association of Naturalists Students of the Jagiellonian University
Метаподаци
Приказ свих података о документуАпстракт:
The highest goal of every species is to survival, reproduce and continue
existence. To fulfill these frogs needs to survive larval and subadult stages
until gain sexual maturity. During this they are exposed to different stressors.
Resistances to external or internal stressors are fitness-related traits that are
central to evolutionary research (Monaghan et al., 2009). One example is
resistance to oxidative stress (OS), which has been suggested to affect lifehistory
trade-offs and fitness (Pamplona and Costantini, 2011). To protect
against oxidative stress, organisms have developed the antioxidative defense
system (AOS), which is comprised of enzymatic (including the activities of
superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx),
glutathione reductase (GR), phase II biotransformation enzyme
glutathione-S-transferase (GST)) and nonenzymatic (the total glutathione
(GSH) contents and sulfhydryl (SH) group concentrations) components. It was
suggested that in younger individuals OS was mainly caused by increased
growing rate and metabolic activity, while in adults it was not only arised
from endogenous metabolism, but also from oxidative challenges induced by
environmental conditions (Metcalfe and Alonso-Alvarez, 2010). In this study we tried to examine AOS of liver of subadult and adult
Bufotes viridis frogs caught from natural population and kept in laboratory
conditions for 14 days, in order to determine possible differences in AOS that
could be related to the frog’s body size and life stage (adults and subadults).
The results showed higher activities of SOD, GR and GST in subadults in
comparison to adults, on the other hand adults had higher activity of GSH-Px
and concentrations of GSH and SH- groups. CAT was the only parameter that
did not differ significantly between groups and did not correlate with frog’s
snout-vent length (SVL). Significant negative correlations were noticed
between frogs SVL and activity of SOD (r=-0.69) and GST (r=-0.45), while
positive correlations of SVL were with GSH-Px (r=0.63), GSH (r=0.67) and SH
groups (r=0.52).
Based on the results we conclude that in subadults enzymatic
components of AOS (SOD, GR and GST) played most important role in
response to OS, while in adults it were nonenzimatic components (GSH and
SH) that were already marked as biomarkers of some xenobiotics that tend to
accumulate.