Up-regulation of ectonucleotidase activity after cortical stab injury in rats
2006
Autori:
Nedeljković, NadeždaBjelobaba, Ivana
Dacić, Sanja
Lavrnja, Irena
Peković, Sanja
Savić, Danijela
Vještica, Aleksandar
Rakić, Ljubisav
Stojiljković, Mirjana
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2006 International Federation for Cell Biology
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 50-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 50-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 50-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 50-nucleotidase demonstrated that CSI induced sig-nificant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 50-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 50-nucleotidase were predominantly astrocytes.
Ključne reči:
ATP; Adenosine; Ectonucleotidase; Ecto NTPDase; Ecto 50-nucleotidase; Cortical stab injury; Rat brainIzvor:
Cell Biology International, 2006, 30, 6, 541-546Finansiranje / projekti:
- Interakcija glije i neurona u procesu oporavka nakon oštećenja centralnog nervnog sistema (RS-MESTD-MPN2006-2010-143005)
DOI: 10.1016/j.cellbi.2006.03.001
PubMed: 16672190
WoS: 000238728600009
Scopus: 2-s2.0-33744773492
URI
https://onlinelibrary.wiley.com/doi/abs/10.1016/j.cellbi.2006.03.001https://radar.ibiss.bg.ac.rs/handle/123456789/3464
https://www.sciencedirect.com/science/article/abs/pii/S1065699506000643