The effects of prenatal dexamethasone exposure and fructose challenge on pituitary-adrenocortical activity and anxiety-like behavior in female offspring
2020
Аутори:
Manojlović-Stojanoski, MilicaNestorović, Nataša
Petković, Branka
Rauš Balind, Snežana
Ristić, Nataša
Trifunović, Svetlana
Ajdžanović, Vladimir
Filipović, Branko
Šošić-Jurjević, Branka
Milošević, Verica
Тип документа:
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Prenatal glucocorticoid overexposure could largely influence pituitary-adrenal activity and anxiety-like behavior in offspring. Our aim was to study the possible potentiating effect of moderate dose of fructose – common ingredient of today’s diet – on prenatal glucocorticoid treatment-induced hypothalamo-pituitary-adrenal (HPA) axis changes. Pregnant female rats were treated with multiple dexamethasone (Dx) doses (3 x 0.5 mg/kg/b.m. Dx; 16th-18th gestational day). Half of female offspring from control and Dx treated dams were supplemented with 10% fructose solution, from weaning till adulthood. Immunohistochemistry, unbiased stereological evaluation and hormonal analysis are used to provide the morpho-functional state of pituitary and adrenal gland. Anxiety-like behavior was assessed using the light/dark box test and the elevated plus maze test. Prenatally Dx exposed females, with or without fructose consumption, had markedly reduced adrenocortical volume (p < 0.05) comparing to controls. Increased basal plasma ACTH level in these females (p < 0.05) maintained corticosterone concentration at control level produced by smaller adrenal glands. In parallel, anxiety-like behavior was shown by both tests used. In conclusion, prenatal Dx exposure cause negative psychophysiological outcome reflected in increased HPA axis activity and anxiety behavior in female offspring, while moderately increased fructose consumption failed to evoke any alteration or to potentiate effects of prenatal Dx exposure.
Напомена:
This is the peer reviewed version of the following article: Manojlović-Stojanoski M, Nestorović N, Petković B, Balind SR, Ristić N, Trifunović S, Ajdžanović V, Filipović B, Šošić-Jurjević B, Milošević V. The effects of prenatal dexamethasone exposure and fructose challenge on pituitary-adrenocortical activity and anxiety-like behavior in female offspring. Tissue Cell. 2020;62:101309. https://doi.org/10.1016/j.tice.2019.101309
Кључне речи:
Adrenal gland; Stereology; Programming; Dexamethasone; FructoseИзвор:
Tissue and Cell, 2020, 62, 101309-Финансирање / пројекти:
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/3494
DOI: 10.1016/J.TICE.2019.101309
ISSN: 0040-8166
PubMed: 32433017
WoS: 000508892200006
Scopus: 2-s2.0-85074229231
URI
https://www.sciencedirect.com/science/article/pii/S0040816619302952?via%3Dihubhttps://radar.ibiss.bg.ac.rs/handle/123456789/3494
https://radar.ibiss.bg.ac.rs/handle/123456789/3495