The vitality of the Serbian spruce ( Picea omorika ) pollen during the long-term cryopreservation
2019
Тип документа:
Чланак у часопису (Рецензирана верзија)
,
© 2019 Collegium Palynologicum Scandinavicum.
Метаподаци
Приказ свих података о документуАпстракт:
This article studies differences in the vitality (germination rate and pollen tube length) of fresh pollen and pollen stored at −20 °C for 14 years of Serbian spruce (Picea omorika [Pančić] Purkyne). Fresh pollen was sampled from 24 trees from a Serbian spruce seed stand area on the site of Bela zemlja (43° 48′ 32″ N, 19° 44′ 25″ E) in the area of Mount Zlatibor (Serbia) in two successive years. Six nutrition media with different sucrose concentrations (0%, 5%, 10%, 15%, 20% and 25% aqueous solution) were used to assess the vitality of the pollen. Variance analysis shows significant differences among years of pollen sampling, years of cryopreservation, trees, and sucrose treatments for pollen germination for both germination rate and pollen tube length. The results indicate that climate conditions preceding the pollen maturation period had an impact on the pollen quality as well as on the pollen vitality during the years of cryopreservation. Inter-individual variability (differences between trees genetic variability) was very pronounced and enabled selection of pollinator trees that retained their initial vitality during the period of cryopreservation. This periodicity in Serbian spruce full flowering might have significant practical application in the breeding of this important species.
Кључне речи:
Cryopreservation; Serbian spruce; Pollen germination; Pollen tube growthИзвор:
Grana, 2019Финансирање / пројекти:
- Истраживање климатских промена и њиховог утицаја на животну средину - праћење утицаја, адаптација и ублажавање (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43007)
DOI: 10.1080/00173134.2019.1668053
WoS: 000494254400001
Scopus: 2-s2.0-85074564194
URI
https://www.tandfonline.com/doi/full/10.1080/00173134.2019.1668053https://radar.ibiss.bg.ac.rs/handle/123456789/3513