Protective effects of Alchemilla vulgaris L. extracts against cisplatin-induced toxicological alterations in rats

Abstract

This study was designed to evaluate the possible effect of methanol extracts of aerial parts and roots of Alchemilla vulgaris L. (AVA and AVR, respectively) in preventing cisplatin-induced hepatorenal and testicular toxicity in rats. UHPLC/DAD/(−)HESI-MS/MS analysis was performed to determine the detailed phenolic profile of AVA and AVR. The male Wistar rats were orally treated with extracts at three different concentrations (50, 100, and 200 mg/kg b.w.) for 10 days and toxicity was induced by injection of single dose of cisplatin on the 5th day (7.5 mg/kg b.w.). Determination of serum biochemical markers of hepatorenal and testicular tissue injury, as well as oxidative-stress parameters in tissues and histopathological study, were performed. Treatments with AVA and AVR significantly attenuated the levels of serum parameters of liver, kidneys and testicles injury, tissue's morphology and parameters of oxidative stress caused by an application of the cisplatin. Chromatographic analysis showed the presence of more than 20 different phenolic compounds in extracts where ellagic acid, catechin, and catechin gallate were dominant components in both extracts. The obtained results indicate that A. vulgaris extracts may be used in preventing cisplatin-induced toxicity during chemotherapy as well as in the treatment of oxidative stress-related disorders.