Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes.
2017
Authors:
Bošnjak-Neumüller, JasnaRadaković, Milena
Djelić, Ninoslav
Vuković-Gačić, Branka
Stevanović, Zora Dajić
Kolarević, Stoimir
Mišić, Danijela
Stanković, Milan
Knežević-Vukčević, Jelena
Spremo-Potparević, Biljana
Stanimirović, Zoran
Document Type:
Article (Published version)
,
© 2019 by the publisher.
Metadata
Show full item recordAbstract:
The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS).
Keywords:
triiodothyronine; DNA damage; Nepeta rtanjensis; phenolic compounds and antigenotoxic activity.Source:
Pakistan Journal of Pharmaceutical Sciences, 2017, 30, 2(Suppl.), 625-634Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-MESTD-Basic Research (BR or ON)-173034)
URI
http://www.ncbi.nlm.nih.gov/pubmed/28650332http://www.pjps.pk/wp-content/uploads/pdfs/30/2/Supplementary/13-SUP-652.pdf
https://radar.ibiss.bg.ac.rs/handle/123456789/3537