Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes.
2017
Autori:
Bošnjak-Neumüller, JasnaRadaković, Milena
Djelić, Ninoslav
Vuković-Gačić, Branka
Stevanović, Zora Dajić
Kolarević, Stoimir
Mišić, Danijela
Stanković, Milan
Knežević-Vukčević, Jelena
Spremo-Potparević, Biljana
Stanimirović, Zoran
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2019 by the publisher.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS).
Ključne reči:
triiodothyronine; DNA damage; Nepeta rtanjensis; phenolic compounds and antigenotoxic activity.Izvor:
Pakistan Journal of Pharmaceutical Sciences, 2017, 30, 2(Suppl.), 625-634Finansiranje / projekti:
- Molekularno-genetička i ekofiziološka istraživanja u zaštiti autohtonih animalnih genetičkih resursa, očuvanju dobrobiti, zdravlja i reprodukcije gajenih životinja i proizvodnji bezbedne hrane (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Aberacije ćelijskog ciklusa i uticaj oksidativnog stresa na neurodegenerativne procese i malignu transformaciju ćelije (RS-MESTD-Basic Research (BR or ON)-173034)
URI
http://www.ncbi.nlm.nih.gov/pubmed/28650332http://www.pjps.pk/wp-content/uploads/pdfs/30/2/Supplementary/13-SUP-652.pdf
https://radar.ibiss.bg.ac.rs/handle/123456789/3537