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Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach
dc.creator | Dragićević, Milan | |
dc.creator | Ćuković, Katarina | |
dc.creator | Zdravković-Korać, Snežana | |
dc.creator | Simonović, Ana | |
dc.creator | Bogdanović, Milica | |
dc.creator | Todorović, Slađana | |
dc.date.accessioned | 2020-01-10T11:22:35Z | |
dc.date.available | 2020-01-10T11:22:35Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0354-4664 | |
dc.identifier.uri | http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900026D | |
dc.identifier.uri | http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4099 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/3568 | |
dc.description.abstract | Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 – GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173015/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Archives of Biological Sciences | |
dc.subject | Germination | |
dc.subject | Glutamine synthetase | |
dc.subject | Grain filling | |
dc.subject | Knockout mutant | |
dc.subject | Phenotype | |
dc.title | Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach | en |
dc.type | article | en |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Драгићевић, Милан; Симоновић, Aна; Ћуковић, Катарина; Здравковић-Кораћ, Снежана; Тодоровић, Слађана; Богдановић, Милица; | |
dc.rights.holder | © 2019 by the Serbian Biological Society. | |
dc.citation.issue | 3 | |
dc.citation.volume | 71 | |
dc.identifier.doi | 10.2298/ABS190315026D | |
dc.identifier.scopus | 2-s2.0-85076490995 | |
dc.identifier.wos | 000491998600007 | |
dc.citation.apa | Dragićević, M., Ćuković, K., Zdravković-Korać, S., Simonović, A., Bogdanović, M., & Todorović, S. (2019). Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach. Archives of Biological Sciences, 71(3), 443–453. | |
dc.citation.vancouver | Dragićević M, Ćuković K, Zdravković-Korać S, Simonović A, Bogdanović M, Todorović S. Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach. Arch Biol Sci. 2019;71(3):443–53. | |
dc.citation.spage | 443 | |
dc.citation.epage | 453 | |
dc.type.version | publishedVersion | |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/5872/ABS-71-3-443-453.pdf | |
dc.citation.rank | M23 |