Trovanje kamforom - spektralna analiza aktivnosti mozga pacova

Abstract

We have decided to detect the seizure brain activity induced by camphor essential oil in rats, as there are still reports of severe intoxication in humans by camphor. The experiments were performed on adult Wistar male rats. The surgery and the acute electrophysiological recordings were done under pentobarbital sodium anesthesia. Local field potentials of cerebral parietal cortex were recorded. The rats were injected by camphor essential oil (150-675 μl/kg b.w., in 1 ml of saline, i.p). We analyzed by Fast Fourier Transform the digitized electrocortical signals before and up 2-3 h after essential oil administration at the sampling rate of 256 Hz. There was a great increase of the mean total power spectra, a relative increase of the power in delta range and decreases of theta and beta ranges when seizural activity with spikes and waves of large amplitude occurred at the level of cerebral cortex 5-30 min after administration of camphor oil at doses >300 μl/kg b.w. It is necessary to investigate further the dose dependent effectiveness of each terpenoid constituent of camphor essential oil on brain activity in order to elucidate the relative safety of the usage of essential oils with these terpenoids.

Odlučili smo se za istraživanje pojave epileptičnih napada kod pacova izazvanih kamforovim esencijalnim uljem jer se još uvek pojavljuju slučajevi teške intoksikacije kamforom u ljudi. Eksperimenti su izvedeni na odraslim pacovima muškog pola Wistar soja. Operativni zahvati i akutna registrovanja elektrofizioloških signala su obavljeni pod Nembutal anestezijom. Registrovani su lokalni potencijali polja parijetalnog dela kore velikog mozga. Kamforovo esencijalno ulje je pacovima intraperitonealno ubrizgavano (150-675 μl/kg, t.m. u l ml fiziološkog rastvora, i.p). Pomoću brze Furijeove transformacije analizirali smo elektrokortikalne signale pre i oko 2-3 h posle intraperitonealnog ubrizgavanja kamforovog ulja, pri frekvenciji semplovanja od 256/s. Pojavio se veliki porast ukupne snage i relativno povećanje snage u delta opsegu, a smanjenje u teta i beta opsegu kada se pojavila epileptična aktivnost kore velikog mozga - šiljci i talasi potencijala velike amplitude i to tek pri dozi kamforovog ulja većoj od 300 μl/kg, a 5-30 min po ubrizgavanju ulja. Neophodno je dalje ispitivati doznu zavisnost efekta svake od glavnih terpenskih komponenti kamforovog ulja na aktivnost mozga pacova kako bi se obezbedila relativna sigurnost upotrebe esencijalnih ulja.