In vitro propagacija Tacitus bellus

Abstract

Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae was discovered in 1972 in a mountainous areas in Mexico and southwest of the USA. Rosettes, up to 10 cm in diameter, lie almost flat to the ground, while large clusters of star like, pink till deep red, flowers appear in May and June, which makes it one of the most fascinating new succulent plants. Sterilized lower rosette leaves were aseptically placed on medium containing, mineral and vitamin solution supplemented with sucrose (3 %), benzylaminopurine (BAP) (2.25 mg dm-3) and naphtalenacetic acid (NAA) (0.186 mg dm-3) arid gelled with agar (0.7 %), Murashige and Skoog, 1962,. Callus and a large number of buds were observed on the explants bases after six months of cultivation on the two-phase medium. The bud multiplications were performed on MS media supplemented with BAP (0.1-0.5 mg dm-3) and NAA (0.1 mg dm-3), while rooting of small rosettes were performed on NAA (0.1 - 0.2 mg dm-3). Spontaneous rooting (80 %) were noticed on hormone free medium while NAA (0.1 or 0.2 mg dm-3) stimulated it up to 100%, depending on clones. Rooted plants were easily acclimatized.

Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae je pronađena 1972 godine u planinskim regionima Meksika i jugozapadne Amerike. Rozete, 10 cm u prečniku, leže gotovo na zemlji. Stabljike sa mnogobrojnim zvezdolikim ružičastim cvetovima, pojavljuju u maju i junu, što ovu biljku čini jednom od najprivlačnijih sukulentnih biljaka. Površinski sterilisani donji listovi rozete su postavljani na podlogu koja sadrži MS mineralni i vitaminski rastvor, saharozu (3 %), BAP (2.25 mg dm-3) i NAA (0.186 mg dm-3) i agar (0.7 %). Posle šest meseci kultivacije na dvofaznom medijumu, primećen je kalus i veliki broj pupoljaka u osnovi eksplantata. Medijumi za multiplikaciju pupoljaka sadržali su BAP (0.1 - 0.5 mg dm-3) i NAA (0.1 mg dm-3), dok su medijumi za ožiljavanje malih rozta sadržali NAA (0.1 ili 0.2 mg dm-3). Spontano ožiljavanje (80 %) je uočeno na podlozi bez hormona, dok je NAA (0.1 i 0.2 mg dm-3) stimulisala ožiljavanje do 100%, u zavisnosti od klona. Ožiljene biljke su lako aklimatizovane.