Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.
2020
Аутори:
Pantić, IgorJeremić, Rada
Dacić, Sanja
Peković, Sanja
Pantić, Senka
Đelić, Marina
Vitić, Zagorka
Brkić, Predrag
Brodski, Claude
Тип документа:
Чланак у часопису (Рецензирана верзија)
,
© Microscopy Society of America 2020.
Метаподаци
Приказ свих података о документуАпстракт:
Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology.
Кључне речи:
Dentate gyrus; Gray-level co-occurrence matrix analysis; Hippocampus; Sensorimotor cortex ablation; Texture; Traumatic brain injuryИзвор:
Microscopy and Microanalysis, 2020, 26, 1, 166-172Финансирање / пројекти:
- Ћелијска и молекулска основа неуроинфламације: потенцијала циљна места за транслациону медицину и терапију (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
- Оптичко микроскопска, имуноморфолошка, молекуларно-биолошка и генетска испитивања малигних и немалигних болести бубрега (RS-MESTD-Basic Research (BR or ON)-175059)
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
- project 92018 of the Mediterranean Society for Metabolic Syndrome, Diabetes and Hypertension in Pregnancy DEGU
DOI: 10.1017/S143192762000001X
ISSN: 1431-9276
PubMed: 31948501