Efikasna regeneracija domaćih sorti krompira (Solanum tuberosum L.- Dragačevka i Jelica) u kulturi in vitro

Abstract

The aim of this study was to find the most suitable protocol for the in vitro regeneration of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragačevka and cv. Jelica), as a prerequisite for further transformation studies. Three different protocols using leaf segments as starting explants were tested. The regeneration efficiency depending on a procedure and a genotype ranged from 41% to 89%, 46% to 92% and from 28% to 57% for Desiree, Dragačevka and Jelica, respectively. The two-step protocol included: (1) the induction of calli on the basal Murashige and Skoog (MS) medium with 0.2 mg l-1 NAA and 2.0 mg l-1 BAP and (2) the regeneration of shoots on the basal medium with 1.5% sucrose, 2.0 mg l-1 BAP and 5 mg l-1 GA3. It was determined that this protocol was the most efficient one for the plant regeneration. This protocol could be successfully applied in the transformation of Dragačevka and Jelica cultivars with Agrobacerium tumfaciens strains. .

U ovom radu su testirana tri protokola za regeneraciju in vitro domaćih sorti krompira dragačevke i jelice iz odsečaka listova. Efikasnost regeneracije je zavisila od genotipa i procedure i iznosila je 41-89% za sortu Desiree, 46-92% za sortu dragačevka i 28-57% za sortu jelica. Dvostepena procedura koja se sastojala iz (1) indukcije kalusa na osnovnoj Murashige and Skoog (MS) hranjivoj podlozi sa 0,2 mg/l NAA i 2,0 mg/l BAP i (2) regeneracije izdanaka na MS podlozi sa 1,5% saharoze, 2,0 mg/l BAP i 5,0 mg/l GA3 se pokazala kao najefikasnija. Ovaj postupak se može uspešno primeniti u transformaciji domaćih kultivara pomoću sojeva Agrobacterium tumfaciens. .