Centaurium erythraea extract reduces redox imbalance and improves insulin expression and secretion in pancreatic β-cells exposed to oxidative and nitrosative stress
2020
Аутори:
Đorđević, MilošGrdović, Nevena
Mihailović, Mirjana
Arambašić Jovanović, Jelena
Uskoković, Aleksandra
Rajić, Jovana
Đorđević, Marija
Tolić, Anja
Mišić, Danijela
Šiler, Branislav
Poznanović, Goran
Vidaković, Melita
Dinić, Svetlana
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Oxidative stress is one of the major mechanisms that underlies the damage of pancreatic b-cells and defects in insulin secretion in diabetes. As herbal preparations can alleviate oxidative stress through their redox-active secondary metabolites, in this study we investigated the cytoprotective effects of Centaurium erythraea extract (CEe) against H2O2- and SNP-induced oxidative/nitrosative stress in Rin-5F b-cells. The antioxidant activity of CEe and its effect on cell survival and insulin expression/secretion were evaluated. The CEe increased cell viability and ameliorated the disturbance of redox homeostasis in H2O2- and SNP-treated cells by decreasing DNA damage, lipid peroxidation and protein S-glutathionylation. The CEe restored GSH homeostasis in H2O2-treated b-cells and attenuated the SNP-induced disturbance of the GSH/GSSG ratio. The H2O2- and SNP-induced disruption of CAT, GPx, GR, MnSOD and CuZnSOD activities was adjusted by the CEe towards control values, as well as mRNA and protein levels of GPx, MnSOD and CAT. The CEe increased insulin expression/secretion particularly in H2O2-treated b-cells, which was in accordance with the more pronounced antioxidant effect of the CEe observed in H2O2-treated b-cells as compared to SNP-treated cells. These findings support the beneficial effect of the CEe in preventing or slowing down b-cell damage and dysfunction caused by oxidative/nitrosative stress during diabetes development.
Кључне речи:
Oxidative stress; Nitrosative stress; β-cells; Centaurium erythraea; Antioxidant; CytoprotectiveИзвор:
Archives of Biological Sciences, 2020, 72, 1, 117-128Финансирање / пројекти:
DOI: 10.2298/abs200127005d
ISSN: 0354-4664
WoS: 000524505000013
Scopus: 2-s2.0-85084220729
URI
http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5029https://radar.ibiss.bg.ac.rs/handle/123456789/3651