Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts
2020
Authors:
Veličković, IvonaŽižak, Željko
Rajčević, Nemanja
Ivanov, Marija
Soković, Marina
Marin, Petar
Grujić, Slavica
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further.
Keywords:
P. spinosa (blackthorn); Antioxidant activity; Antimicrobial activity; Antidiabetic activity; α-glucosidase inhibitory activitySource:
Archives of Biological Sciences, 2020, 72, 1, 105-115Funding / projects:
- Micromorphological, phytochemical and molecular investigations of plants - systematic, ecological and applicative aspects (RS-MESTD-Basic Research (BR or ON)-173029)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- Biological response modifiers in physiological and pathological conditions (RS-MESTD-Basic Research (BR or ON)-175011)
DOI: 10.2298/abs191217004v
ISSN: 0354-4664
WoS: 000524505000012
Scopus: 2-s2.0-85084237331
URI
http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4932https://radar.ibiss.bg.ac.rs/handle/123456789/3652