Antioxidant and antimicrobial activity of two Asplenium species
2020
Authors:
Živković, SuzanaMilutinović, Milica
Maksimović, Vuk
Ćirić, Ana
Ivanov, Marija
Božunović, Jelena
Banjanac, Tijana
Mišić, Danijela
Document Type:
Article (Published version)
,
© 2020 SAAB
Metadata
Show full item recordAbstract:
Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH• and ABTS•+), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(–)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.
Keywords:
Asplenium; Ferns; Biofilm; Phenolic compoundsSource:
South African Journal of Botany, 2020, 132, 180-187Funding / projects:
- Application of low temperature plasmas in biomedicine, environmental protection and nanotechnologies (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41011)
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Physics of nanostructured oxide materials and strongly correlated systems (RS-MESTD-Basic Research (BR or ON)-171032)
- The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-MESTD-Basic Research (BR or ON)-173040)
DOI: 10.1016/j.sajb.2020.03.034
ISSN: 0254-6299