Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
2020
Authors:
Cvetić Antić, TijanaJanošević, Dušica
Maksimović, Vuk M.
Živić, Miroslav
Budimir, Snežana
Glamočlija, Jasmina
Mitrović, Aleksandra Lj
Document Type:
Article (Published version)
,
© 2019 Elsevier GmbH.
Metadata
Show full item recordAbstract:
We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
Keywords:
Fungal leaf infection; Fusaric acid; Plant antioxidative system; Stomata; Transition from biotrophy to necrotrophySource:
Journal of Plant Physiology, 2020, 244, 153086-Funding / projects:
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-MESTD-Basic Research (BR or ON)-173017)
- Biotechnology in vitro - crop, medicinal and endangered plant species (RS-MESTD-Basic Research (BR or ON)-173015)
- The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-MESTD-Basic Research (BR or ON)-173040)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
DOI: 10.1016/j.jplph.2019.153086
ISSN: 1618-1328
PubMed: 31812905