Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro
2020
Authors:
Ćosić, TatjanaSavić, Jelena
Raspor, Martin
Cingel, Aleksandar
Ghalawnji, Nabil
Vinterhalter, Branka
Ninković, Slavica
Document Type:
Article (Accepted Version)
Metadata
Show full item recordAbstract:
Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of
the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators
(PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were
studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength
MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose
and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA),
gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all
treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a
distinctive manner, with a variety of morphological traits being altered in comparison to matching
controls.
Keywords:
Kohlrabi tissue culture; Sugars; Plant growth regulators; Morphogenesis, de novo shoot formationSource:
Archives of Biological Sciences, 2020, 72, 3, 349-357Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.2298/ABS200622029C
ISSN: 0354-4664