Aloe emodin inhibits the cytotoxic action of tumor necrosis factor
2007
Autori:
Harhaji-Trajković, LjubicaMijatović, Sanja
Maksimović-Ivanić, Danijela
Popadić, Dusan
Isaković, Aleksandra
Todorović-Marković, Biljana
Trajković, Vladimir
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2007 Elsevier B.V.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
We demonstrate the capacity of an herbal anthraquinone aloe emodin to reduce the cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNF) towards L929 mouse fibrosarcoma and U251 human glioma cell lines. Aloe emodin inhibited both TNF-induced cell necrosis and apoptosis, but it did not reduce cell death induced by UV radiation or hydrogen peroxide. Aloe emodin inhibited both basal and TNF-triggered activation of extracellular signal-regulated kinase (ERK), and a selective blockade of ERK activation mimicked the cytoprotective action of the drug. On the other hand, aloe emodin did not affect TNF-induced activation of p38 mitogen-activated protein kinase or generation of reactive oxygen species. The combination of aloe emodin and TNF caused an intracellular appearance of acidified autophagic vesicles, and the inhibition of autophagy with bafilomycin or 3-methyladenine efficiently blocked the cytoprotective action of aloe emodin. These data indicate that aloe emodin could prevent TNF-triggered cell death through mechanisms involving induction of autophagy and blockade of ERK activation.
Ključne reči:
Aloe emodin; Tumor necrosis factor; Cytotoxicity; Apoptosis; Necrosis; Autophagy; Extracellular signal-regulated kinaseIzvor:
European Journal of Pharmacology, 2007, 568, 1-3, 248-259Finansiranje / projekti:
- Fiziološka i farmakološka modulacija imunoinflamatornih i malignih bolesti (RS-MESTD-MPN2006-2010-143029)
- Mehanizmi urođene i stečene imunosti u autoimunskim bolestima i infekciji (RS-MESTD-MPN2006-2010-145066)
DOI: 10.1016/j.ejphar.2007.04.029
ISSN: 0014-2999
PubMed: 17531219