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Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation.
dc.creator | Krstić Milošević, Dijana | |
dc.creator | Banjac, Nevena | |
dc.creator | Janković, Teodora | |
dc.creator | Eler, Klemen | |
dc.creator | Vinterhalter, Branka | |
dc.date.accessioned | 2020-09-03T11:14:31Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0981-9428 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/32763798 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/123456789/3847 | |
dc.description.abstract | Shoot and root in vitro culture of endemic European species Gentiana clusii was established for the first time. The effects of different concentrations of benzyl adenine (BA), 6-phurphurylaminopurine (KIN), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on shoot propagation and rooting of G. clusii were investigated. The optimal in vitro conditions for shoot propagation and long-term maintenance were achieved using woody plant medium (WPM) supplemented with 0.5 mg l-1 KIN, and subsequent application of IBA at 0.5 mg l-1 significantly improved rooting of these shoots. Root culture was established from excised root tips cultured in ½ MS liquid media with increasing concentrations of IBA (0.1-1.0 mg l-1). A high root growth rate and considerable biomass yield were obtained by addition of 1.0 mg l-1 IBA. HPLC analysis revealed that in vitro culture considerably promoted the production of secondary metabolites in G. clusii. The selected protocol for shoot propagation (WPM + 0.5 mg l-1 KIN) increased the content of sweroside, gentiopicrin and norswertianin-1-O-primeveroside (N-1-P) for more than 2-fold compared with the wild plants. IBA promoted N-1-P and norswertianin production in root cultures; their contents were enhanced 6.4- and 18.6-fold, respectively, compared with the wild plants. The extract of these roots displayed the highest antioxidant capacity (IC50 = 66.57 μg ml-1). The established shoot and root propagation protocols facilitate in vitro conservation of G. clusii, and provides a promising tool for the large scale production of valuable secoiridoids and xanthones. | en |
dc.publisher | Elsevier Masson SAS | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | |
dc.rights | restrictedAccess | |
dc.source | Plant Physiology and Biochemistry | |
dc.subject | Antioxidant activity | |
dc.subject | HPLC analysis | |
dc.subject | Plant growth regulators | |
dc.subject | Secoiridoids | |
dc.subject | Xanthones | |
dc.title | Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation. | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Крстић Милошевић, Дијана; Елер, Клемен; Бањац, Невена; Јанковић, Теодора; Винтерхалтер, Бранка; | |
dc.rights.holder | © 2020 Elsevier Masson SAS | |
dc.citation.volume | 154 | |
dc.identifier.doi | 10.1016/j.plaphy.2020.07.007 | |
dc.identifier.pmid | 32763798 | |
dc.identifier.scopus | 2-s2.0-85088870774 | |
dc.identifier.wos | 000566818900069 | |
dc.citation.apa | Krstić-Milošević, D., Banjac, N., Janković, T., Eler, K., & Vinterhalter, B. (2020). Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation. Plant Physiology and Biochemistry, 154, 735–744. | |
dc.citation.vancouver | Krstić-Milošević D, Banjac N, Janković T, Eler K, Vinterhalter B. Gentiana clusii Perr.&Song.: Enhanced production of secondary metabolites by in vitro propagation. Plant Physiol Biochem. 2020;154:735–44. | |
dc.citation.spage | 735 | |
dc.citation.epage | 744 | |
dc.type.version | publishedVersion | |
dc.citation.rank | M21 |