Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.
2020
Преузимање 🢃
Аутори:
Lunić, Tanja M.Oalđe, Mariana M.
Mandić, Marija R.
Sabovljević, Aneta D
Sabovljević, Marko S.
Gašić, Uroš
Duletić-Laušević, Sonja N.
Božić, Bojan Dj.
Božić Nedeljković, Biljana Dj.
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Recently, there has been an increasing interest in the chemistry and biological potential of mosses, since a large number of biologically active compounds have been found within these species. This study aimed at examining the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory/antineurodegenerative, and antitumor activities) of moss Hypnum cupressiforme Hedw. extracts. Corresponding extracts have been obtained applying Soxhlet extractor. The chemical characterization was performed using spectrophotometric assays and liquid chromatography-mass spectrometry (LC-MS). The extracts were analyzed for antioxidant activity and for inhibitory activities on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase. Additionally, extracts were tested against four cell lines-MRC-5, BV2, HCT-116, and MDA-MB-231-for antitumor and anti-inflammatory activities. Chemical analysis of extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. Major compounds identified by LC-MS in H. cupressiforme were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. According to biochemical assays the investigated extracts exhibited significant immunomodulatory potential. Significant antiproliferative potential against MDA-MB-231 cells has been observed together with the promising anti-neuroinflammatory application. The obtained data suggest that moss H. cupressiforme is a valuable natural source of biologically active compounds with potential application in the pharmaceutical industry.
Кључне речи:
Hypnum cupressiforme; Anti-neuroinflammatory/antineurodegenerative; Antidiabetic; Antioxidant; Antitumor activity; Moss extractИзвор:
Molecules (Basel, Switzerland), 2020, 25, 15, 3343-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
DOI: 10.3390/molecules25153343
ISSN: 1420-3049
PubMed: 32717985
WoS: 000567095900001
Scopus: 2-s2.0-85088810618
URI
https://www.mdpi.com/1420-3049/25/15/3343http://www.ncbi.nlm.nih.gov/pubmed/32717985
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7436104
https://radar.ibiss.bg.ac.rs/123456789/3851