In vitro propagation of Catalpa ovata G. Don and Catalpa Bignonioides Walt.

Abstract

Shoot cultures of Catalpa ovata and Catalpa bignonioides were started from epicotyls of aseptically germinated seeds and maintained on Murashige and Skoog (1962) based medium. Optimal BA concentration for shoot multiplication was 0.5 mg/l for Catalpa ovata nad 1.0 mg/l for Catalpa bignonioides. IBA had little effect on shoot multiplication and 0.1 mg/l was considered optimal for both species. Shoots of Catalpa bignonioides were more then twice longer than shoots of Catalpa ovata on all BA concentrations exceeding 0.1 mg/l. Shoots 1.5 - 2.0 cm long could be easily rooted, media supplemented with 0.5 mg/l IBA was considered optimal. Rooted plantlets were adapted in glasshouse after planting in mixtures of peat, sand and organic manure.