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Partial characterization of a lipase from gypsy moth (Lymantria dispar L.) larval midgut
dc.creator | Mrdaković, Marija | |
dc.creator | Lazarević, Jelica | |
dc.creator | Perić Mataruga, Vesna | |
dc.creator | Ilijin, Larisa | |
dc.creator | Vlahović, Milena | |
dc.date.accessioned | 2020-10-28T14:09:06Z | |
dc.date.available | 2020-10-28T14:09:06Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 0015-5497 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/3931 | |
dc.description.abstract | Lipase activity of the gypsy moth (Lymantria dispar L.) was studied by the spectrophotometric method using crude homogenate of fifth-instar larval midgut tissues as the enzyme source and p-nitrophenyl caprylate (pNPC) as substrate. A Km value of 0.310mM and a Vmax value of 1.479U/mg prot. were obtained for this substrate. Among various p-nitrophenyl esters tested, maximum activity was obtained for p-nitrophenyl caprylate and p-nitrophenyl caprate. The enzyme was most active at alkaline pH, with maximum at pH 8.2. Decreased activity was detected after preincubation in buffers of pH below 7.0 and above 8.2. The enzyme was unstable at room temperature. The enzyme was Ca2+ independent. Its activity was inhibited by PMSF, Fe2+, Ag+ and Pb2+, while Fe3+ inhibited enzyme activity by about 40%. | en |
dc.language.iso | en | sr |
dc.publisher | Institute of Systematics and Evolution of Animals, Polish Academy of Sciences | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/143033/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Folia biologica (Kraków) | sr |
dc.subject | Lymantria dispar L. | sr |
dc.subject | lipase | sr |
dc.subject | p-nitrophenyl esters | sr |
dc.subject | optimal conditions | sr |
dc.subject | inhibition | sr |
dc.title | Partial characterization of a lipase from gypsy moth (Lymantria dispar L.) larval midgut | en |
dc.type | article | sr |
dc.rights.license | BY | sr |
dcterms.abstract | Лазаревић, Јелица; Мрдаковић, Марија; Влаховић, Милена; Илијин, Лариса; Перић-Матаруга, Весна; | |
dc.rights.holder | © 2008 by Institute of Systematics and Evolution of Animals, Polish Academy of Sciences | sr |
dc.citation.issue | 1-2 | |
dc.citation.volume | 56 | |
dc.identifier.doi | 10.3409/fb56_1-2.103-110 | |
dc.identifier.pmid | 19055033 | |
dc.identifier.scopus | 2-s2.0-38349129641 | |
dc.identifier.wos | 000252377100016 | |
dc.citation.spage | 103 | |
dc.citation.epage | 110 | |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/7503/Mrdakovic-et-al-2008-Folia-biologica.pdf | |
dc.citation.rank | M23 |