Selektivni inhibitorni efekat retinoida RO 10-9359 na pojedine faze proliferacije T limfocita indukovane lektinima

Abstract

The in vitro effects of aromatic retinoid RO 10-9359 on rat T lymphocyte proliferative response to lectins were evaluated. A decrease of PHA- and Con A-induced proliferation was observed in the presence of increasing amounts (0.05-50 (Jg/ml) of RO 10- 9359. In experiments designed to test the inhibitory effects of the drag with respect to specific inducible events which regulate lymphocyte mitogenesis, it was shown that RO 10-9359 did not inhibit the early events of T cell triggering, because it did not interfere with the production of IL-1 by macrophages, nor did it affect IL-1 dependent events of T cell activation, i. e., the acquisition of IL-2 receptors and the IL-2 production. Neither was the la-antigen expression altered by RO 10-9359. In contrast, the ability of T cells that had previously acquired IL-2 receptors to respond to exogenously supplied IL-2, was reduced in a dose-dependent way. These results suggest that RO 10-9359 primarily affects the later phase of proliferative activity after T cell triggering, which is initiated by the interaction of IL-2 with its receptors and which is IL-1 independent.