Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats
2020
Authors:
Elaković, IvanaKovačević, Sanja
Vojnović-Milutinović, Danijela
Nikolić-Kokić, Aleksandra
Glban, Alhadi M.
Spasić, Mihajlo
Tappy, Luc
Đorđević, Ana
Matić, Gordana
Brkljačić, Jelena
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
The effects of early-life fructose consumption on hepatic signaling pathways and their relation to the development of metabolic disorders in later life are not fully understood. To investigate whether fructose over consumption at a young age induces alterations in glucocorticoid signaling that might contribute to development of metabolic disturbances, we analysed glucocorticoid receptor hormone-binding parameters and expression of its target genes involved in gluconeogenesis (phosphoenolpyruvate carboxykinase and glucose-6-phosphatase) and lipid metabolism (lipin-1),as well as redox and inflammatory status in the liver of female rats subjected to a fructose-rich diet immediately after weaning. The fructose diet increased hepatic corticosterone concentration,11β-hydroxysteroid dehydrogenase type 1 level, glucocorticoid receptor protein level and hormone-binding activity, as well as lipin-1 level. The expression of glucose-6-phosphatase was reduced in fructose-fed rats, while phosphoenolpyruvate carboxykinase remained unaltered.The fructose-rich diet increased the level of fructose transporter GLUT2, while the expression of fructolytic enzymes fructokinase and aldolase B remained unaltered. The diet also affected pro-inflammatory pathways, but had no effect on the antioxidant defence system. In conclusion, a fructose-rich diet applied immediately after weaning promoted lipogenesis and enhanced hepatic glucocorticoid signaling, possibly to protect against inflammatory damage, but without an effect on gluconeogenesis and antioxidant enzymes. Yet, prolonged treatment might ultimately lead to more pronounced metabolic disturbances.
Keywords:
glucocorticoid receptor; inflammation; antioxidant enzymes; lipogenesis; fructose-fed rat; lipin-1Source:
Nutrients, 2020, 12, 11, 3470-Funding / projects:
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
DOI: 10.3390/nu12113470
ISSN: 2072-6643