Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling
2020
Authors:
Bijelić, Dunja D.Milićević, Katarina D.
Lazarević, Milica
Miljković, Đorđe
Bogdanović Pristov, Jelena J.
Savić, Danijela
Petković, Branka
Anđus, Pavle R.
Momčilović, Miljana
Nikolić, Ljiljana
Document Type:
Article (Accepted Version)
,
© 2020 Wiley Periodicals LLC
Metadata
Show full item recordAbstract:
Interaction between autoreactive immune cells and astroglia is an important part of the pathologic processes that fuel neurodegeneration in multiple sclerosis. In this inflammatory disease, immune cells enter into the central nervous system (CNS) and they spread through CNS parenchyma, but the impact of these autoreactive immune cells on the activity pattern of astrocytes has not been defined. By exploiting naïve astrocytes in culture and CNS-infiltrated immune cells (CNS IICs) isolated from rat with experimental autoimmune encephalomyelitis (EAE), here we demonstrate previously unrecognized properties of immune cell-astrocyte interaction. We show that CNS IICs but not the peripheral immune cell application, evokes a rapid and vigorous intracellular Ca2+ increase in astrocytes by promoting glial release of ATP. ATP propagated Ca2+ elevation through glial purinergic P2X7 receptor activation by the hemichannel-dependent nucleotide release mechanism. Astrocyte Ca2+ increase is specifically triggered by the autoreactive CD4+ T-cell application and these two cell types exhibit close spatial interaction in EAE. Therefore, Ca2+ signals may mediate a rapid astroglial response to the autoreactive immune cells in their local environment. This property of immune cell-astrocyte interaction may be important to consider in studies interrogating CNS autoimmune disease.
Note:
This is the peer reviewed version of the following article: Bijelić DD, Milićević KD, Lazarević MN, Miljković DM, Bogdanović Pristov JJ, Savić DZ, Petković BB, Andjus PR, Momčilović MB, Nikolić LM. Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling. J Neurosci Res. 2020;98(11):2317-32, which has been published in final form at http://dx.doi.org/10.1002/jnr.24699. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
Keywords:
RRID:AB_10013382; RRID:AB_10050580; RRID:AB_1018856; RRID:AB_10682518; RRID:AB_162542; RRID:AB_162543; RRID:AB_2224402; RRID:AB_2534013; RRID:AB_2534102; RRID:AB_2535792; RRID:AB_2538778; RRID:AB_2629482; RRID:AB_2828023; RRID:RGD_21409748; RRID:RGD_21409752; RRID:SCR_002285; RRID:SCR_002798; RRID:SCR_003210; RRID:SCR_011323; RRID:SCR_014235; Astrocytes; Calcium signaling; Cell communication; Nervous system autoimmune disease; Purinergic receptorsSource:
Journal of Neuroscience Research, 2020, 98, 11, 2317-2332Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- Fellowship Start up for Science from the Centre for Leadership Development to Ljiljana M. Nikolić
Related info:
DOI: 10.1002/jnr.24699
ISSN: 0360-4012
PubMed: 32799373
WoS: 000563900600001
Scopus: 2-s2.0-85089392101
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/jnr.24699http://www.ncbi.nlm.nih.gov/pubmed/32799373
https://radar.ibiss.bg.ac.rs/123456789/3859
https://radar.ibiss.bg.ac.rs/handle/123456789/4006