Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro
2020
Authors:
Đurić, MarijaSubotić, Angelina
Prokić, Ljiljana
Trifunović-Momčilov, Milana
Cingel, Aleksandar
Vujičić, Milorad
Milošević, Snežana
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.
Keywords:
Abiotic stress; Abscisic acid; Antioxidants; Impatiens walleriana; Molecular analysisSource:
Plants, 2020, 9, 11, 1559-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/plants9111559
ISSN: 2223-7747
PubMed: 33202704