Trout farm effect on antioxidative defense in Dinocras megacephala (Plecoptera: Perlidae) larvae
2014
Аутори:
Bjelanović, KatarinaŽivić, Ivana
Stojanović, Dalibor
Todorović, Dajana
Mirčić, Dejan
Filipović, Aleksandra
Perić Mataruga, Vesna
Остала ауторства
Mauchart, PéterCsabai, Zoltán
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2014 by the University of Pécs, Medical School Press
Метаподаци
Приказ свих података о документуАпстракт:
Trout production represents one of the major agricultural activities in the first and
second order streams of Serbia. Organic compounds are drained into the
environment usually without previous sedimentation and thus affect stream
biota. Since biological monitoring is commonly based on aquatic
macroinvertebrates, we used Dinocras megacephala (Klapálek, 1907) larvae as
the target organism in order to estimate trout farm effects on physiological stress.
Four localities were chosen at the channel of the Raska River (in the vicinity of
Novi Pazar, Serbia), two upstream localities and the other two downstream from
the trout farm outlet. Specimens were collected with tweezers and placed in
liquid nitrogen for further analysis. Basic physical and chemical water parameters
were measured directly in the field: temperature, dissolved oxygen, pH and
conductivity, and in the laboratory: total phosphorus (TP), orthophosphates (OP)
and ionized ammonia (NH4+). Even though the temperature stayed quite constant
along the water course (11.1°C to 11.2°C), oxygen concentration decreased from
the reference localities (10.4 mg/l and 11 mg/l) to the downstream localities (7
mg/l and. 8 mg/l). Also, the concentration of ionized ammonia was almost ten
times higher at the same localities (from 0.0319 and 0.0288 mg/l to 0.3141 and
0.2423 mg/l). The activity of superoxide dismutase (SOD), catalase (CAT) and the
total glutathione amount (GSH) were analyzed in order to determine the level of
oxidative stress caused by the increase of organic compounds originating from the
trout farm. The activity of SOD and CAT enzymes was significantly higher in the
samples collected the both downstream localities. Total glutathione amount
(GSH) decreases in the specimens collected at the first downstream locality, while
the level of enzyme activity at the second downstream locality coincides with the
samples from both upstream localities. The results indisputably indicate that
higher concentration of organic compounds from the trout farm induce the
significant increase of the oxidative stress in the examined species.
Финансирање / пројекти:
- Утицај магнетних поља и других срединских стресора на физиолошке одговоре и понашање различитих врста (RS-MESTD-Basic Research (BR or ON)-173027)
У:
- Mauchart P, Csabai Z, editors Book of abstracts & programme: 1st Central European Symposium for Aquatic Macroinvertebrate Research; 2014 Apr 10‐13; Szarvas, Hungary. Pécs, Hungary: University of Pécs, Medical School Press; 2014. p. 33.