Motor behaviour and energy metabolism of Blaptica dubia in artificial magnetic fields
2019
Аутори:
Petković, BrankaIlijin, Larisa
Todorović, Dajana
Mrdaković, Marija
Vlahović, Milena
Grčić, Anja
Perić Mataruga, Vesna
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2019 by RAD Centre
Метаподаци
Приказ свих података о документуАпстракт:
During the evolution, all organisms are exposed to the constant action of different environmental
factors, biotic and abiotic, which are variable in time and space. Life on Earth is formed in a natural
magnetic field (geomagnetic field). Therefore, magnetic field (MF) is classified as abiotic factor that
help maintain different life processes of plants, animals and humans, as well as their biological and
physiological functions. Organisms are exposed to new type of artificial MFs, due to the increasing
electrification and technological development. So, MF is growing environmental pollutant and as a
consequence of response and/or adaptation of organisms on new, stressful conditions, changes could
be observed on all levels of the biological organization. So, the aim of this study was to evaluate the
long – term effect of a static MF (SMF) and an extremely low frequency MF (ELF MF) on the motor
behaviour in Blaptica dubia nymphs, as well as on their fat body glycogen concentration and total
lipid content. One month old nymphs were randomly divided into three experimental groups:
control, exposed to SMF (110 mT) and exposed to ELF MF (50 Hz, 10 mT). The cockroaches were
exposed to these MFs for 5 months, while the control ones were kept outside the reach of the
magnetic field. Fat body glycogen concentration, as well as total lipid content, was measured
spectrophotometrically according to Wyatt and Kalf (1957) and Stone and Mordue (1980),
respectively. Also, we monitored nymphal behaviour for 10 minutes in “open – field” test and
analyzed several behavioural parameters (travel distance, average speed while in motion, time
mobile, travel distance of the head, number of body rotations, immobility time) using ANY – maze
software. Exposure to SMF and ELF MF affected the all examined behavioural parameters. Namely,
in comparison to control cockroaches, all parameters, except immobility time, were significantly
higher in B. dubia nymphs exposed to both MFs. The difference between the MF groups was no
statistically significant. In SMF and ELF MF groups, fat body glycogen concentration was
significantly lower compared to control group. Between the MF groups significant differences were
not observed. The lipid concentration in the fatty body of B. dubia nymphs depended on the applied
MF. In nymphs exposed to SMF, total lipid content was significantly higher compared to control. ELF
MF caused its decrease compared to control and SMF group. These differences were significant only
for SMF group. This study provides evidences that long – term exposure of B. dubia nymphs to
magnetic fields induces important alterations in their motor behaviour, and consequently examined
parameters of carbohydrate and lipid metabolism.
Финансирање / пројекти:
- Утицај магнетних поља и других срединских стресора на физиолошке одговоре и понашање различитих врста (RS-MESTD-Basic Research (BR or ON)-173027)
У:
- Seventh International Conference on Radiation in Various Fields of research - RAD 7 : book of abstracts. 2019 Jun 10-14; Herceg Novi, Montenegro. Niš, Serbia: RAD Centre; 2019. p. 77.