Antimikrobna aktivnost etarskog ulja juniperus communis l.

Abstract

Composition of the essential oil of Juniperus communis L. and its potential antimicrobial activity were analyzed. Composition of the essential oil was analysed by GC and GC/MS. The major components were a-pinene (40.33%), b-myrcene (13.72%), limonene (5.61%) and sabinene (5.38%). As test organisms the following micromycetes Penicillium ochrochloron, Aspergillus niger, Trichoderma viride and bacteria Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli and Micrococcus luteus were used. Microdilution method on microplates 96-system was used for detection of minimum inhibition concentration (MIC), minimum bactericidal (MBC) and fungicidal concentration (MFC). Essential oil did not show antibacterial activity against E. coli, and for B. subtilis and M. luteus it was low. The oil of J. communis exhibited low antifungal activity against tested micromycetes.

U ovom radu je analiziran hemijski sastav etarskog ulja obične kleke (Juniperus communis L.) i njegovo antimikrobno (antibakterijsko i antifungalno) dejstvo. Za utvrđivanje sastava etarskog ulja kleke korišćeni su GC i GC/MS. Najzastupljenije komponente u ovom ulju su a-pinen (40.33%), b-mircen (13.72%), limonen (5.61%) i sabinen (5.38%). Test organizmi korišćeni u eksperimentu su bile mikromicete: Penicillium ochrochloron, Aspergillus niger, Trichoderma viride i bakterijski sojevi Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli i Micrococcus luteus. Metodom mikrodilucije na mikrotitracionim pločama, utvrđivane su minimalne inhibitorne i mikrobicidne koncentracije (MIC i MMC). Etarsko ulje je pokazalo aktivnost na ispitivane mikromicete na višim koncentracijama. Etarsko ulje je na B. subtilis i M. luteus pokazalo slabiju aktivnost dok na bakteriju E. coli nije delovalo.