In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes
2015
Преузимање 🢃
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The tall glandular trichomes occurring on young expanding leaves of Nicotiana tabacum grown in
vitro were investigated using light and electron microscopy. Previous studies reported that the tall
glandular trichomes of tobacco produce large quantities of various compounds including diterpenes.
To explore the cellular structures required and putatively involved in diterpene biosynthesis and
secretion, the present study focused on the development of the tall glandular trichomes, and on
histochemical and ultrastructural analysis of their secretory cells under in vitro conditions. Electron
microscopy confirmed earlier light microscopy observations of the glandular nature of the tall leaf
trichomes. Their head cells, which secrete resinous material, exhibited characteristics common
to gland cells: a dense cytoplasm, numerous mitochondria and little vacuolation. They contained
structurally well-developed chloroplasts and an elaborate network of endoplasmic reticulum, as
well as electron dense inclusions in chloroplasts and in small vacuoles. The dominance of smooth
endoplasmic reticulum suggests its involvement in diterpene biosynthesis. Histochemical tests were
positive for both lipophilic and hydrophilic secretions, which included terpenoids (essential oils
and resins) and acidic/neutral lipids, as well as phenolic compounds (tannins), alkaloids, mucilage/
pectin and polysaccharides.
Кључне речи:
Glandular trichomes; Histochemistry; Morphology; Tobacco; UltrastructureИзвор:
Botanica Serbica, 2015, 39, 2, 103-110Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)