Genotoxicity of Fluoride Subacute Exposure in Rats and Selenium Intervention
2020
Аутори:
Radovanović, JelenaAntonijević, Biljana
Kolarević, Stoimir
Milutinović-Smiljanić, Sanja
Mandić, Jelena
Vuković-Gačić, Branka
Bulat, Zorica
Ćurčić, Marijana
Kračun-Kolarević, Margareta
Sunjog, Karolina
Kostić-Vuković, Jovana
Jovanović Marić, Jovana
Antonijević-Miljaković, Evica
Đukić-Ćosić, Danijela
Djordjevic, Aleksandra Buha
Javorac, Dragana
Baralić, Katarina
Mandinić, Zoran
Тип документа:
Чланак у часопису (Објављена верзија)
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© 2020 Elsevier Ltd
Метаподаци
Приказ свих података о документуАпстракт:
The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140–190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.
Кључне речи:
Comet assay; DNA damage; NaF; Subacute toxicity; Wistar ratsИзвор:
Chemosphere, 2020, 128978-Финансирање / пројекти:
DOI: 10.1016/j.chemosphere.2020.128978
ISSN: 0045-6535
PubMed: 33298328