Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy
2020
Аутори:
Miler, IrenaRabasović, Mihailo D.
Aleksić, Marija
Krmpot, Aleksandar J.
Kalezić, Anđelika
Janković, Aleksandra
Korać, Bato
Korać, Aleksandra
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2020 Wiley-VCH GmbH
Метаподаци
Приказ свих података о документуАпстракт:
Our previous study on rat skin showed that cumulative oxidative pressure induces profound structural and ultrastructural alterations in both rat skin epidermis and dermis during aging. Here, we aimed to investigate the biophotonic properties of collagen as a main dermal component in the function of chronological aging. We used second harmonic generation (SHG) and two‐photon excited fluorescence (TPEF) on 5 μm thick skin paraffin sections from 15‐day‐, 1‐month‐ and 21‐month‐old rats, respectively, to analyze collagen alterations, in comparison to conventional light and electron microscopy methods. Obtained results show that polarization‐resolved SHG (PSHG) images can detect collagen fiber alterations in line with chronological aging and that this method is consistent with light and electron microscopy. Moreover, the β coefficient calculated from PSHG images points out that delicate alterations lead to a more ordered structure of collagen molecules due to oxidative damage. The results of this study also open the possibility of successfully applying this fast and label‐free method to previously fixed samples.image
Кључне речи:
Chronological aging; Collagen; Electron microscopy; Light microscopy; Polarization‐resolved SHG imaging; Second‐harmonic generation microscopy; Two‐photon excited fluorescence microscopyИзвор:
Journal of Biophotonics, 2020Финансирање / пројекти:
- Ministry of Education, Science and Technological Development of the Republic of Serbia.
DOI: 10.1002/jbio.202000362
ISSN: 1864-063X
PubMed: 33231371
WoS: 000595066900001
Scopus: 2-s2.0-85096957346
URI
https://onlinelibrary.wiley.com/doi/10.1002/jbio.202000362https://radar.ibiss.bg.ac.rs/handle/123456789/4072
Институција/група
IBISSAPA:
Miler, I., Rabasovic, M. D., Aleksic, M., Krmpot, A. J., Kalezic, A., Jankovic, A., et al. (2020). Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy. Journal of Biophotonics, DOI:10.1002/jbio.202000362.Vancouver:
Miler I, Rabasovic MD, Aleksic M, Krmpot AJ, Kalezic A, Jankovic A, Korac B, Korac A. Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy. J Biophotonics. 2020;DOI:10.1002/jbio.202000362.Related items
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