Somatic embryogenesis in Centaurium erythraea Rafn - current status and perspectives: a review
2021
Аутори:
Simonović, AnaTrifunović-Momčilov, Milana
Filipović, Biljana
Marković, Marija
Bogdanović, Milica
Subotić, Angelina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum
of secondary metabolites with confirmed healing properties. Centaury is an emerging model in
plant developmental biology due to its vigorous regenerative potential and great developmental
plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic
embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions
to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic
embryos. When SE is initiated from centaury root explants, the process occurs spontaneously
(on hormone-free medium), directly (without the callusing phase), and the somatic embryos are
of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is
indirect (preceded by callusing). Histological observations and culture conditions are compared
in these two systems. The changes in antioxidative enzymes were followed during SE from the
leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were
analyzed using a variety of approaches. The newest and preliminary results, including centaury
transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed
as perspectives of centaury research.
Кључне речи:
Antioxidative enzymes; Arabinogalactan proteins; Centaury; Gentianaceae; In vitro culture; Morphogenesis; Plant growth regulators; Somatic embryo; Tissue cultureИзвор:
Plants, 2021, 10, 70-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/plants10010070
ISSN: 2223-7747
PubMed: 33396285