Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress
2021
Аутори:
Ilijin, LarisaGrčić, Anja
Mrdaković, Marija
Vlahović, Milena
Filipović, Aleksandra
Matić, Dragana
Perić Mataruga, Vesna
Тип документа:
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт:
In this paper the effects of increased environmental temperature on the relative growth rate (RGR) and developmental time in 5th instar L. dispar larvae originating from unpolluted and polluted forests were analyzed. As indicators of the level of generated reactive oxygen species in thermal stress, we estimated midgut and hemolymph activity of the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT), as well as the detoxifying enzymes glutathione S-transferase (GST), carboxylesterase (CaE) and acetylcholinesterase (AChE) from the midgut and brain tissue. We also examined the influence of induced thermotolerance as a species’ ability to overcome the negative effects of this stressor. In larvae originating from the unpolluted forest, the midgut is the primary location of increased SOD and CAT activity and induced thermotolerance did not modified their activity in either tissue. In larvae from the polluted forest, in both tissues SOD activity was more sensitive to an increased temperature and induced thermotolerance than CAT. Carboxylesterase responded diversely to thermal stress depending on the analyzed tissue regardless the origin of larvae, while the activity of GST and AChE in tissue depended on the origin of larvae. Induced thermotolerance modified the activity of detoxifying enzymes in larvae originating from the polluted forest. Combining the selected parameters into an integrated biomarker response (IBR) the GST, CaE and AChE battery emerged as a potential biomarker for thermal stress in L. dispar larvae.
Напомена:
This is the peer reviewed version of the following article: Ilijin L, Grčić A, Mrdaković M, Vlahović M, Filipović A, Matić D, Mataruga VP. Tissue-specific responses of Lymantria dispar L. (Lepidoptera: Erebidae) larvae from unpolluted and polluted forests to thermal stress. J Therm Biol. 2021;96:102836. http://dx.doi.org/10.1016/j.jtherbio.2021.102836
Related to: https://radar.ibiss.bg.ac.rs/handle/123456789/4083
Кључне речи:
Thermal stress; Different larval tissues; Antioxidative enzymes; Detoxifying enzymes; Larvae from unpolluted and polluted forestsИзвор:
Journal of Thermal Biology, 2021, 96, 102836-Финансирање / пројекти:
DOI: 10.1016/j.jtherbio.2021.102836
ISSN: 0306-4565
WoS: 000640910400004
Scopus: 2-s2.0-85099190019
URI
https://radar.ibiss.bg.ac.rs/handle/123456789/4083https://radar.ibiss.bg.ac.rs/handle/123456789/4089