Prenatal treatment with metronidazole induces cerebellar folia alteration in guinea pig fetuses
2020
Authors:
Čapo, IvanMilenković, Ivan
Čapo, Nataša
Stilinović, Nebojša
Vukmirović, Saša
Teofilovic, Branislava
Petković, Branka
Sekulić, Slobodan
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
The most sensitive period in brain development is during prenatal life. The use of antibiotics in pregnancy is still controversial. Recent studies revealed the high neurotoxic potential of the antibiotic and antiprotozoal medication, metronidazole. However, there are insufficient data from animal studies about prenatal treatment effects. We investigated the effect of prenatal treatment with metronidazole on cerebellar development in guinea pigs. Treatment with metronidazole was performed from the 42nd to the 49th day of gestation. On the 50th day of pregnancy, all dams were killed, and the cerebella of the fetuses were analyzed. Gross cerebellar changes characterized by malposition of the folia with partial atrophy were found in 12 of 19 fetuses in the experimental group, but in none of 20 control fetuses that received saline. The most affected were folia VII with depletion of the areal fraction of the external granular layer, molecular layer and the internal granular layer. Purkinje cells displayed cell distortion with loss of normal dendritic polarity. The investigation revealed cell depletion, with a disturbance of the cytoarchitectonic of the cerebellar cortex and folia alteration.
Keywords:
Cerebellum; Foliation; Metronidazole; Neuronal migration; NeurotoxicitySource:
Archives of Biological Sciences, 2020, 72, 4, 473-482Funding / projects:
- The effect of prenatal exposure to antiepileptic therapy on the neurological development (RS-MESTD-Basic Research (BR or ON)-175006)
- Autonomous Province of Vojvodina, Republic of Serbia, Grant No: 142-451-3176/2020-01
DOI: 10.2298/ABS200619041C
ISSN: 0354-4664
WoS: 000604979700003
Scopus: 2-s2.0-85099165190
URI
http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642000041Chttps://radar.ibiss.bg.ac.rs/handle/123456789/4102
http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6035