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Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study
dc.creator | Miler, Marko | |
dc.creator | Ajdžanović, Vladimir | |
dc.creator | Živanović, Jasmina | |
dc.creator | Marković Filipović, Jelena | |
dc.creator | Šošić-Jurjević, Branka | |
dc.creator | Milošević, Verica | |
dc.date.accessioned | 2021-02-18T11:58:29Z | |
dc.date.available | 2022-02-15 | |
dc.date.issued | 2021 | |
dc.identifier.issn | 1431-9276 | |
dc.identifier.uri | https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/abs/thyroid-gland-alterations-in-oldaged-wistar-rats-a-comprehensive-stereological-ultrastructural-hormonal-and-gene-expression-study/4C35B9A6AFED404C65272C08C8C2DC27 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/4143 | |
dc.description.abstract | The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation, presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin (Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles. Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression of the thyroid gland synthetic and secretory capacity with advanced age. | sr |
dc.language.iso | en | sr |
dc.publisher | Cambridge University Press | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Microscopy and Microanalysis | sr |
dc.subject | NIS | sr |
dc.subject | T4-Tg | sr |
dc.subject | Tg | sr |
dc.subject | TPO | sr |
dc.subject | TSH | sr |
dc.title | Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study | sr |
dc.type | article | sr |
dc.rights.license | BY | sr |
dcterms.abstract | Живановић, Јасмина; Милер, Марко; Aјджановић, Владимир; Милошевић, Верица; Марковић Филиповић, Јелена; Шошић-Јурјевић, Бранка; | |
dc.rights.holder | © 2021 by the author(s). Published by Cambridge University Press on behalf of the Microscopy Society of America. | sr |
dc.identifier.doi | 10.1017/S1431927621000064 | |
dc.identifier.pmid | 33586646 | |
dc.identifier.scopus | 2-s2.0-85100831764 | |
dc.identifier.wos | 000700414000021 | |
dc.citation.apa | Miler, M., Ajdžanović, V., Živanović, J., Marković Filipović, J., Šošić-Jurjević, B., & Milošević, V. (2021). Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. Microscopy and Microanalysis, DOI:10.1017/S1431927621000064. | |
dc.citation.vancouver | Miler M, Ajdžanović V, Živanović J, Marković Filipović J, Šošić-Jurjević B, Milošević V. Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. Microsc Microanal. 2021;DOI:10.1017/S1431927621000064. | |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/8217/thyroid-gland-alterations-in-old-aged-wistar-rats-a-comprehensive-stereological-ultrastructural-hormonal-and-gene-expression-study.pdf | |
dc.citation.rank | M21 |