The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers
2021
Аутори:
Li, HanluoMasieri, Federica Francesca
Schneider, Marie
Bartella, Alexander
Gaus, Sebastian
Hahnel, Sebastian
Zimmerer, Rüdiger
Sack, Ulrich
Maksimović-Ivanić, Danijela
Mijatović, Sanja
Simon, Jan-Christoph
Lethaus, Bernd
Savković, Vuk
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.
Кључне речи:
Hair follicle; In vitro cultivation; Mesenchymal stem cells; Multipotency; Neuroectodermal cells; Noninvasive sampling; Outer root sheathИзвор:
Biomolecules, 2021, 11, 2, 154-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Deutsche Forschungsgemeinschaft
- Sächsisches Staatsministerium für Wissenschaft und Kunst
DOI: 10.3390/biom11020154
ISSN: 2218-273X
PubMed: 33503918
WoS: 000622118300001
Scopus: 2-s2.0-85100116195
URI
internal-pdf://Li et al. - 2021 - The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem.pdfhttps://www.mdpi.com/2218-273X/11/2/154
https://radar.ibiss.bg.ac.rs/handle/123456789/4149