Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.
2021
Аутори:
Drača, DijanaEdeler, David
Saoud, Mohamad
Dojčinović, Biljana
Dunđerović, Duško
Đmura, Goran
Maksimović-Ivanić, Danijela
Mijatović, Sanja
Kaluđerović, Goran N.
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2021 Elsevier Inc.
Метаподаци
Приказ свих података о документуАпстракт:
CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4',6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2',7'-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP.
Кључне речи:
Apoptosis; Autophagy; Cisplatin; Cytotoxicity; Drug carrier; SBA-15Извор:
Journal of Inorganic Biochemistry, 2021, 217, 111383-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
DOI: 10.1016/j.jinorgbio.2021.111383
ISSN: 0162-0134
PubMed: 33582397
WoS: 000632487200001
Scopus: 2-s2.0-85100813341
URI
internal-pdf://Drača et al. - 2021 - Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells i.pdfhttps://linkinghub.elsevier.com/retrieve/pii/S0162013421000301
http://www.ncbi.nlm.nih.gov/pubmed/33582397
https://radar.ibiss.bg.ac.rs/handle/123456789/4154