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Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.
dc.creator | Drača, Dijana | |
dc.creator | Edeler, David | |
dc.creator | Saoud, Mohamad | |
dc.creator | Dojčinović, Biljana | |
dc.creator | Dunđerović, Duško | |
dc.creator | Đmura, Goran | |
dc.creator | Maksimović-Ivanić, Danijela | |
dc.creator | Mijatović, Sanja | |
dc.creator | Kaluđerović, Goran N. | |
dc.date.accessioned | 2021-02-26T13:58:19Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0162-0134 | |
dc.identifier.uri | internal-pdf://Drača et al. - 2021 - Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells i.pdf | |
dc.identifier.uri | https://linkinghub.elsevier.com/retrieve/pii/S0162013421000301 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/33582397 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/4154 | |
dc.description.abstract | CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4',6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2',7'-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP. | |
dc.publisher | Elsevier BV | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Inorganic Biochemistry | |
dc.subject | Apoptosis | |
dc.subject | Autophagy | |
dc.subject | Cisplatin | |
dc.subject | Cytotoxicity | |
dc.subject | Drug carrier | |
dc.subject | SBA-15 | |
dc.title | Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies. | |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Дојчиновић, Биљана; Дунђеровић, Душко; Максимовић-Иванић, Данијела; Мијатовић, Сања; Ђмура, Горан; Саоуд, Мохамад; Калуђеровић, Горан Н.; Драча, Дијана; Еделер, Давид; Aнтитумор потентиал оф цисплатин лоадед инто СБA-15 месопороус силица нанопартицлес агаинст Б16Ф1 меланома целлс: ин витро анд ин виво студиес.; | |
dc.rights.holder | © 2021 Elsevier Inc. | |
dc.citation.volume | 217 | |
dc.identifier.doi | 10.1016/j.jinorgbio.2021.111383 | |
dc.identifier.pmid | 33582397 | |
dc.identifier.scopus | 2-s2.0-85100813341 | |
dc.identifier.wos | 000632487200001 | |
dc.citation.apa | Drača, D., Edeler, D., Saoud, M., Dojčinović, B., Dunđerović, D., Đmura, G., et al. (2021). Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies. Journal of Inorganic Biochemistry, 217, 111383. | |
dc.citation.vancouver | Drača D, Edeler D, Saoud M, Dojčinović B, Dunđerović D, Đmura G, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN. Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies. J Inorg Biochem. 2021;217:111383. | |
dc.citation.spage | 111383 | |
dc.type.version | publishedVersion | |
dc.citation.rank | M21 |