The discovery, distribution and diversity of DNA viruses associated with Drosophila melanogaster in Europe
2021
Аутори:
Wallace, Megan ACoffman, Kelsey A
Gilbert, Clément
Ravindran, Sanjana
Albery, Gregory F
Abbott, Jessica
Argyridou, Eliza
Bellosta, Paola
Betancourt, Andrea J
Colinet, Hervé
Eric, Katarina
Glaser-Schmitt, Amanda
Grath, Sonja
Jelić, Mihailo
Kankare, Maaria
Kozeretska, Iryna
Loeschcke, Volker
Montchamp-Moreau, Catherine
Ometto, Lino
Onder, Banu Sebnem
Orengo, Dorcas J
Parsch, John
Pascual, Marta
Patenković, Aleksandra
Puerma, Eva
Ritchie, Michael G
Rota-Stabelli, Omar
Schou, Mads Fristrup
Serga, Svitlana V
Stamenković-Radak, Marina
Tanasković, Marija
Savić Veselinović, Marija
Vieira, Jorge
Vieira, Cristina P
Kapun, Martin
Flatt, Thomas
González, Josefa
Staubach, Fabian
Obbard, Darren J
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This deficiency limits our opportunity to use natural host-pathogen combinations in experimental studies, and may bias our understanding of the Drosophila virome. Here we report fourteen DNA viruses detected in a metagenomic analysis of approximately 6500 pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new nudiviruses, a new and divergent entomopoxvirus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of galbut virus, a dsRNA partitivirus, segregating at very low frequency. Remarkably, we find that Drosophila Vesanto virus, a small DNA virus previously described as a bidnavirus, may be composed of up to 12 segments and thus represent a new lineage of segmented DNA viruses. Two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, found in 2% or more of wild flies. The others are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus the only ones commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redressing an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.
Кључне речи:
DNA virus; Drosophila; Endogenous viral element; Adintovirus; Bidnavirus; Densovirus; Filamentous virus; Galbut virus; NudivirusИзвор:
Virus Evolution, 2021Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- UK Natural Environmental Research Council through the E3 doctoral training programme (NE/L002558/1)
- Wellcome Trust PhD programme (108905/Z/15/Z)
- BBSRC grant BB/P00685X/1
- Swiss National Science Foundation grants 31003A-182262, PP00P3_165836, and PP00P3_133641/1
- Agence Nationale de la Recherche (grant ANR-15-CE32-0011-01)
- H2020-ERC-2014-CoG-647900
- Fundación Española para la Ciencia y la Tecnologia-Ministerio de Economía y Competitividad (FCT-15-10187)
- Deutsche Forschungsgemeinschaft grant GR 4495/2
- Academy of Finland projects 268214 and 322980
- Austrian Science Fund (FWF) grant P32275
- Danish Research council for natural Sciences (FNU) grant nr 4002-00113B
- Scientific and Technological Research Council of Turkey (TUBITAK) (Grant No. 214Z238)
- Deutsche Forschungsgemeinschaft grant PA 903/8
- Deutsche Forschungsgemeinschaft grant STA1154/4-1; Projektnummer 408908608
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
- Special Topics Network (STN) grant by the European Society of Evolutionary Biology (ESEB)
URI
https://academic.oup.com/ve/advance-article/doi/10.1093/ve/veab031/6207981https://radar.ibiss.bg.ac.rs/handle/123456789/4184