Complete Freund's adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies.
2021
Authors:
Lazarević, MilicaNikolovski, Neda
Stanisavljević, Suzana
Dimitrijević, Mirjana
Stegnjaić, Goran
Krishnamoorthy, Gurumoorthy
Mostarica Stojković, Marija
Miljković, Đorđe
Jevtić, Bojan
Document Type:
Article (Accepted Version)
Metadata
Show full item recordAbstract:
Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8+CD11bc+MHC class II+ cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model.
Note:
This is the peer reviewed version of the following article: Lazarević M, Djedovic N, Stanisavljević S, Dimitrijević M, Stegnjaić G, Krishnamoorthy G, Mostarica Stojković M, Miljković Đ, Jevtić B. Complete Freund’s adjuvant-free experimental autoimmune encephalomyelitis in Dark Agouti rats is a valuable tool for multiple sclerosis studies. J Neuroimmunol. 2021;354:577547. http://dx.doi.org/10.1016/j.jneuroim.2021.577547
Keywords:
CD4(+) T cells; CD8(+) macrophages; CFA; EAESource:
Journal of Neuroimmunology, 2021, 354, 577547-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- European Research Council starting grant (GAMES; 635617)
- German research foundation (DFG) SFB TR-128 (Project A1)
- Max Planck Society
Related info:
- Referenced by
https://radar.ibiss.bg.ac.rs/handle/123456789/4193
DOI: 10.1016/j.jneuroim.2021.577547
ISSN: 0165-5728
PubMed: 33765502