Antagonistic interaction between phosphinothricin and nepeta rtanjensis essential oil affected ammonium metabolism and antioxidant defense of arabidopsis grown in vitro
2021
Authors:
Dmitrović, SlavicaDragićević, Milan
Savić, Jelena
Milutinović, Milica
Živković, Suzana
Maksimović, Vuk
Matekalo, Dragana
Perišić, Mirjana
Mišić, Danijela
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days expo-sure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.
Keywords:
Ammonium toxicity; Antagonism; Antioxidant defense; Arabidopsis; BASTA; Essential oil; Glutamine synthetase; Nepeta; PhosphinothricinSource:
Plants, 2021, 10, 1, 1-20Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
DOI: 10.3390/plants10010142
ISSN: 2223-7747
PubMed: 33445496