Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
2021
Аутори:
Stupar, PetarPodolski-Renić, Ana
Villalba, Maria Ines
Dragoj, Miodrag
Jovanović Stojanov, Sofija
Pešić, Milica
Kasas, Sandor
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer world-wide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability.
Кључне речи:
Atomic force microscope; Cancer cells; Cantilever; Multidrug resistance; Nano-motion; Personalized therapyИзвор:
Medicina, 2021, 57, 5, 446-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/medicina57050446
ISSN: 1010-660X
PubMed: 34064439
WoS: 000654312800001
Scopus: 2-s2.0-85106628163
URI
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147836/https://radar.ibiss.bg.ac.rs/handle/123456789/4247