Sensitivity of midgut physiological parameters of Lymantria dispar L. larvae to benzo[a]pyrene in populations with different multigeneration contact to environmental pollutants
2021
Аутори:
Grčić, AnjaIlijin, Larisa
Matić, Dragana
Filipović, Aleksandra
Mrdaković, Marija
Todorović, Dajana
Perić Mataruga, Vesna
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Accumulation of organic pollutants in the environment calls for sensing physiological parameters adequate to
indicate the presence of contaminants and their effects on ecosystems. Evidence points to the importance of
insect adaptations in their habitats for the assessment of sensitive biomarkers so we examined the influence of
origin and multigenerational adaptations of the Lymantria dispar larvae to chronic benzo[a]pyrene (B[a]P)
treatment under laboratory conditions. The main aim was to compare reactions of larvae from unpolluted and
polluted forests using alkaline phosphatase (ALP), acid phosphatase (ACP), and carboxylesterase (CE) specific
activities in the midgut, including electrophoretic isoform patterns; midgut expression levels of Hsp70, larval
development time (DT), and midgut mass (MM), after chronic exposure to 5 and 50 ng of B[a]P/g dry food
weight. The biomarker potential of these parameters regarding larval pre-exposure history to pollution was
estimated by principal component analysis (PCA). B[a]P treatment resulted in inhibition of ALP activity, a rise of
CE activity, and reduction of MM in larvae from the unpolluted forest, while the population from the polluted
forest showed significant elevation of Hsp70 expression in the midgut, prolonged DT, and reduction of MM. PCA
confirmed variations in responses of the selected parameters regarding population origin. The obtained results
provide insight into insect population variability concerning physiological responses to pollutants. It is indicative
that all investigated physiological parameters of L. dispar larvae showed origin-dependent responses to long-term
presence of B[a]P, which may be of great importance in ecotoxicological research.
Кључне речи:
Phosphatase; benzo[a]pyrene; Carboxylesterase; Hsp70; Lymantria dispar; PCAИзвор:
Environmntal Pollution, 2021, 288, 117706-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 10.1016/j.envpol.2021.117706
ISSN: 0269-7491