dc.contributor | Uzelac, Branka | |
dc.creator | Lukić, Milana | |
dc.creator | Skorić, Marijana | |
dc.creator | Matekalo, Dragana | |
dc.creator | Božunović, Jelena | |
dc.creator | Aničić, Neda | |
dc.creator | Filipović, Biljana | |
dc.creator | Mišić, Danijela | |
dc.date.accessioned | 2021-09-01T12:59:42Z | |
dc.date.available | 2021-09-01T12:59:42Z | |
dc.date.issued | 2018 | |
dc.identifier.isbn | 978-86-912591-4-3 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/4290 | |
dc.description.abstract | Centaurium erythraea (centaury), a plant with a long tradition of medicinal use, contains bioactive
secoiridoid glucosides (SGs). The exogenous application of methyl jasmonate (MeJA) may
elicit a response similar to stress, and is used in the present study to induce SG accumulation and
expression of SGs’ biosynthesis associated genes in centaury’s aerial parts. UHPLC-MS/MS analyses
have indicated that the addition of 250 μM MeJA to the medium has caused overproduction of
almost all investigated secondary metabolites. The most notable change was detected for swertiamarin
production, which was several fold-higher in MeJA-treated plants. For the purpose of determining
the key genes of MeJA response, we performed a qPCR analysis of all genes potentially
involved in the secoiridoid biosynthetic pathway. After five days of MeJA treatment, an increase
of relative gene expression was detected for G8O, 8HGO7, IO, 7DLGT, 7DLH2, SLS and CPR1. On the
other hand, in centaury plants subjected to MeJA treatment for ten days, there was no significant
increase in gene expression. For GPPS and LAMT, decreased expression levels were detected, when compared to control plants. Patterns of SGs biosynthetic gene expression are highly correlated to
SGs’ profiles during MeJA-elicitation treatments, giving clear evidence that the identified genes
are a part of SGs’ biosynthetic routes. All the results indicate that MeJA is a suitable elicitor that
leads, through gene expression changes, to increased flux through the pathway and production
of swertiamarin, sweroside and gentiopicrin, which can be used for future production of secoiridoids
with the aid of biotechnological processes. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade: Serbian Plant Physiology Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts | sr |
dc.subject | Centaurium erythraea | sr |
dc.subject | gene expression | sr |
dc.subject | methyl jasmonate | sr |
dc.subject | secoiridoid glucosides | sr |
dc.subject | UHPLC-MS/MS analysis | sr |
dc.title | Elicitation effects of methyl jasmonate on secoiridoid production in shoot culture of centaury (Centaurium erythraea Rafn) | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dcterms.abstract | Aничић, Неда; Мишић, Данијела; Лукић, Милана; Скорић, Маријана; Матекало, Драгана; Божуновић, Јелена; Филиповић, Биљана; | |
dc.rights.holder | © 2018 by the Serbian Plant Physiology Society | sr |
dc.description.other | Uzelac B, editor. Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology; 2018. p. 118-9. | sr |
dc.citation.spage | 118 | |
dc.type.version | publishedVersion | sr |
dc.identifier.cobiss | 264421900 | |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/8742/3-IntConfPlantBiology_2018-118-119.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_4290 | |